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  • Cell Culture

    •  D.Mel2, Leishmania

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  • DNA

    • Isolate: Genomic DNA

    • Resolve and Recover:

      • Agarose Gel Electrophoresis (<25-kb)

      • Pulsed-Field Gel Electrophoresis (>25kb DNA)

    • ​Analysis​

      • Southern blotting

      • Plaque hybridization

      • DNA sequencing (Sanger)

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  • RNA

    • Total RNA (TRIzol™ Reagent)

    • Northern blotting

    • RT-PCR               

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  • PCR

    • Primer and Degenerate primer design

    • Polymerase Chain Reaction (PCR),          

  • Cloning

    • Isolate Plasmid DNA

    • Restriction Enzyme Digestion

    • Ligation

    • E.coli transformation

    • Screening Recombinant Plasmids​

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  • Bioinformatics

    • BlastX

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